by Hanyayu Niu
2026,8(2);
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Abstract
Bacterial biofilms are often discussed as a secondary problem in the conservation of old
buildings, but on damp or dust-laden surfaces they can become an active part of material decay. Here, 72
surface samples from historic buildings in Foshan, Guangzhou, and Shanxi/Taiyuan were compared to
examine how much of the bacterial pattern is shared across regions and how much is shaped by individual
buildings and substrates. The sample set covered a humid subtropical setting in southern China and a
temperate monsoon setting in northern China, and included wood, tile, brick, wall, statue, and cave-wall
surfaces. Community profiles were generated from 16S rRNA amplicon sequencing and interpreted with
taxonomic summaries, rarefaction analysis, PCA, Bray-Curtis PCoA, PERMANOVA, and FAPROTAX-based functional prediction. Four phyla, Proteobacteria, Actinobacteriota, Firmicutes, and Bacteroidota,
together made up more than 80% of the reads in each region, suggesting that these surfaces carry a broad
but recognizable bacterial core. At genus or species level the picture was less uniform. Pseudomonas,
Sphingomonas, Acinetobacter, and Bacillus appeared repeatedly, yet their abundance changed with
building context and surface material. Ordination results pointed in the same direction: The separation
among buildings and substrates was clearer than the separation among broad geographic regions, and
PERMANOVA confirmed significant compositional differences (p
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